ChIP seq

The fellows just met in Madrid on the week of the 18th-22nd of September to learn how to perform a ChIP-seq on Brassica napus. They first went in-depth into the theory and then they went hands-on the protocol.

But… What is ChIP-seq?

ChIP-seq is a tool to investigate the epigenomes of multiple cell types and their underlying mechanisms. It is based on chromatin immunoprecipitation (ChIP) which is a protein-DNA binding in vivo assay technique. The ChIP antibodies are specific to our protein or nucleosome of interest. ChIP is followed by sequencing (the –seq part of ChIP-seq), which allows to identify and sequence our enriched DNA fragments (Park, 2009).

There are four basic steps for ChIP, as you can see in the image below. Firstly, crosslinking is the way to improve the connection between protein and chromatin to preserve the interaction for further antibody targeting. There are two common methods to do crosslinking, using formaldehyde or ultraviolet (UV) light. For this ChIP-seq course, we used 1% formaldehyde. After crosslinking, we need to extract chromatin, and shearing/sonicate them. Shearing step to solubilize the chromatin which is very important to get enough chromatin yield. In addition, a proper shearing strategy to generate desirable DNA size (300bp-500bp) is good for immunoprecipitation and signal detection. The next step is to remove these fragments without the connection between the targeted protein and chromatin. To achieve this, we need to use antibodies to bind with targeted proteins where the antibody can stick to magnetic beads. So, the non-target proteins can be washed out, followed by eluting the targeted protein from the beads. Then, de-crosslinking means removing the proteins from the DNA to make the purified DNA accessible easily. Lastly, indexing barcodes will be added to the purified DNA for individual sample identification. If the quality control test shows positive results, the library could be used for ChIP-seq.

 

(Daniel et al., 2014)

From this we understand how ChIP sequencing is carried out and the steps involved in successful preparation of a library for sequencing, further using various sequencing platforms sequencing of the prepared libraries can be carried out.

With excellent guidance by Dr. Pedro Crevillén and Dr. Jose A. Abelenda, fellows of the EpiSeedLink doctoral network were skillfully trained in ChIP sequencing. This will immensely help each of the fellows in the next phase of their PhD journey, where they will incorporate the knowledge gained in practical terms.

Each fellow will use ChIP sequencing technique to uniquely benefit their research project, resulting in generating enormous data that will help the research community in understanding the dynamics of both Arabidopsis and Brassica napus genome.

 

Text by Shreyas Padmanabha Sharma Beedubail, PhD Student EpiSeedLink Marie Skłodowska-Curie Actions

 

References:

  1. Daniel, B., Balint, B. L., Nagy, Z. S., & Nagy, L. (2014). Mapping the genomic binding sites of the activated retinoid X receptor in murine bone marrow-derived macrophages using chromatin immunoprecipitation sequencing. Steroid Receptors: Methods and Protocols, 15-24.
  2. Park, P. J. (2009). ChIP–seq: advantages and challenges of a maturing technology. Nature reviews genetics10(10), 669-680.

 

 

Frontal image by <a href="https://www.freepik.es/foto-gratis/especialista-biotecnologia-laboratorio-realizando-experimentos_44133702.htm#query=ChIP%20seq%20DNA%20protein&position=0&from_view=search&track=ais">Freepik</a>


The moon, the plants, and the humans

How the story started 

When it was proposed to do science outreach material as part of the EpiseedLink program, my first thought was to talk about curiosities about plants. Then, I asked my friends and family what they wanted to know about plants or which questions they had about plants. Then, my brother came up with a very interesting story.

In Brazil, we have a martial art called Capoeira, which mixes dance, music, and fighting. This martial art surged during the colonial period of the country and was developed by Africans brought to Brazil as slaves. Capoeira is closely related to the resistance of Africans and African descendants against slavery and the fight against racism. Nowadays, it is one of the symbols of Brazilian culture.

Capoeira uses different musical instruments and one of them is the berimbau. This instrument is mainly made of the wood of a tree called Biriba. When he was a Capoeira student, my brother heard his master saying that to make the instrument berimbau, you need to cut the tree in the evening, during the new moon or waning moon. The reason is that the probability of the wood rotting is lower if cut during this period.

This kind of knowledge is known as traditional knowledge, a result of observation, technologies, and practices developed by indigenous people, and passed down generation by generation. As a scientist, this made me very curious. How does the moon affect plant development? What do we know about this? Which other cultures made similar observations and have similar practices? Inspired by this story, I started my investigation to answer these questions.

 

The moon and its effect on animals

The most known effect of the moon on our planet is the tide. The gravity force of the moon pushes the water of oceans, increasing the water level. This happens twice a day, in a cycle known as the tidal cycle. The level of the seas increases more when the moon and the sun are aligned during the full and new moon. The gravitational force of these two celestial bodies sums up, generating what we call the spring tides.  Also, the different moon phases generate differences in the amount of light during the night, in a cycle of around 30 days.

These differences in gravity and light due to the moon are detected by animals. For example, research indicates that the spawn of corals is dependent on moonlight intensity. Different species of nightjars follow the moon phases in their nesting period. The females of a crab species in Japan go down the mountain to lay their eggs during spring tides. These and other examples show the influence of the moon on animals' reproduction, physiology, and behavior.

 

 

The gravity effect on plants

What about the plants? One of the most known effects of gravitational force on plants being studied is the diameter of the trees and leaf movement. Under the natural variation of the light of day and night, the diameter of a tree increases more during the day, in a cycle of around 24h. However, if you put the tree in constant light, the diameter of the tree increases in a cycle of around 25h. This cycle is coincident with the variance in the local gravitational force. This local gravitational force is the result of the sum of the gravitational force of the moon and the sun, called the lunisolar gravitational force. It is supposed that when this force increases, it pulls the water in the tree, like the tides in the ocean.

As expected, the movement of the water in the trunk and the diameter of the truck seem to be correlated to the lunisolar gravitational force. It was also identified that the leaf movement of beans synchronized with the lunisolar gravitational force. If the plant can perceive the gravitational force, if there is a molecular mechanism associated with this, and how deep the effect of the lunisolar gravitational force is in the plant growth and development still needs to be investigated.

 

The light effect on plants

Even though the light of the moon is not enough to keep the plant alive, substituting the sun, this light seems to be detected by the plants. The moonlight is the result of the sunlight being reflected by the moon. However, the composition of these two lights is not the same. The moonlight is composed mainly of blue light in very low energy, while sunlight is composed of a long range of wavelengths at a high energy level. Then, it is expected that plants do not detect moonlight and sunlight equally.

The study of Breitler and collaborators investigates the detection of moonlight by plants from a molecular perspective. They discover that from the different proteins that detect light, known as photoreceptors, only one is expressed during full moon compared to new moon. They also detect many other genes that are affected by moonlight, having differences in their expression. What are the observable effects of the moonlight beyond the molecular effect must still be investigated. But since the moonlight affects animals, plants that are directly affected by animal predation, for example, may also use the moonlight as a cue.

 

The moon phases effect on plants

In Central America, more specifically Belize, Panama and Puerto Rico, indigenous people have the tradition of harvesting natural resources in the full moon. They perceive that the palm leaves collected during the full moon, for example, are more durable than the ones collected in other periods. These palm leaves are harvested for different reasons, one of the uses is to construct the rooftop of houses.

The study of Vogt and collaborators focuses on this tradition and tries to associate the full moon with the period when herbivores act more. Plants do not let themselves be consumed by herbivores passively. They defend themselves by producing chemical compounds. These chemical compounds can increase the durability of the plant and their survival in front of herbivores. What the scientists did was quantify chemical compounds associated with plant defense and durability in different moon phases. They identified that the concentration of some of the compounds quantified changed according to the moon phase. In the end, they suggest that plants produce more compounds against herbivores during the full moon. These compounds also help the durability of the leaves. The indigenous people, therefore, started to perceive that the durability increased during this period because the plant was defending itself from herbivores.

 

 

Conclusion

When to sow, when to harvest, when to cut the wood. Besides the period to cut the birimba to construct the musical instrument used in Capoeira or when to cut palm leaves to construct houses, a range of traditional human practices are correlated to the moon. Like the light and the temperature, the moon has a predictable cycle, inputting light and gravitational cues to the plants. Humans in different parts of the world observed this, giving rise to diverse traditional practices passed down from generation to generation in different human societies. Some of these practices were and are being studied by scientists. We are still in the initial stage of understanding how it all works, but gradually, the influence of the moon over plants will be unveiled.

 

Author: Maira Marins Dourado, PhD Student EpiSeedLink Marie Skłodowska-Curie Actions

Reviewed by: Ana Paula Avelino dos Santos

Curious? These are some of the references used in this article, that you may find interesting:

About traditional knowledge:

https://press.un.org/en/2019/hr5431.doc.htm

About Capoeira:

https://www.smithsonianmag.com/smithsonian-institution/capoeira-occult-martial-art-international-dance-180964924/

About human traditions, plants, and the moon:

http://www.soin-de-la-terre.org/wp-content/uploads/PlantsandtheMoon.pdf

About tides:

https://moon.nasa.gov/moon-in-motion/earth-and-tides/tides/#:~:text=Spring%20tides%20always%20happen%20when,it%20perpendicular%20to%20the%20Sun.

About animals and the moon:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712431/

About lunisolar gravity and plants:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215047/

About moonlight and plants:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961272/

About secondary metabolites and the moon:

https://www.jstor.org/stable/4315291

A recent review about the plants and the moon:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8866634/

Other references:

https://pubmed.ncbi.nlm.nih.gov/21002732/

http://www.soin-de-la-terre.org/wp-content/uploads/PlantsandtheMoon.pdf

https://link.springer.com/article/10.1007/s00709-010-0136-6

https://www.researchgate.net/profile/Ernst-Zuercher/publication/250209219_Tree_stems_and_tides_A_new_approach_and_elements_of_reflexion_reviewed_paper/links/585d50b108ae6eb8719ff5ad/Tree-stems-and-tides-A-new-approach-and-elements-of-reflexion-reviewed-paper.pdf

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529214/#CIT0116

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215041/

https://www.liebertpub.com/doi/epdf/10.1089/ast.2006.6.668

<a href="https://www.freepik.es/foto-gratis/hombres-entrenando-capoeira-playa_34305440.htm">Imagen de pikisuperstar</a> en Freepik

 


Arabidopsis thaliana vs. Brassica napus L.: A Tale of Two Cruciferous Crops!

Did you know that both, Arabidopsis thaliana and Brassica napus L., have taken center stage in the innovative EpiSeedLink project?

Arabidopsis thaliana and Brassica napus L. are two fascinating members of the Brassicaceae family, with distinctive characteristics and roles in the plant world. Let's explore their unique features and contributions!

 

 

🌿 Arabidopsis thaliana:

Commonly known as "rockcress," Arabidopsis thaliana is a model plant used extensively in scientific research. Its small size, typically reaching about 10-20 centimeters in height, and short lifecycle of about 6-8 weeks under optimal conditions make it ideal for studying plant genetics, development, and responses to environmental factors. Arabidopsis thaliana serves as a powerful tool in understanding fundamental processes in plants and has contributed significantly to advancements in biotechnology.

 

🥬 Brassica napus L. (Canola/Rapeseed):

Brassica napus L. is an essential oilseed crop widely cultivated for its high-quality vegetable oil, known as "canola oil." This versatile crop has diverse applications, from culinary use to industrial purposes, such as biofuel production. Brassica napus L. has a more extended growth cycle compared to Arabidopsis thaliana, around 5-6 months from planting to maturity making it an excellent candidate for studying agricultural practices and crop improvement.

 

 

Together, Arabidopsis thaliana and Brassica napus L. are igniting a new era of plant research through the EpiSeedLink project. By delving deep into their genomes and epigenomes, we hope to pave the way for exciting discoveries that will benefit global agriculture and ensure a more sustainable future.

 

Text by Kingsley Onyinye Ibeabuchi, PhD Student EpiSeedLink Marie Skłodowska-Curie Action

The frontal image about the differences between the two species by Shannon Skye Derman, PhD Student Marie Skłodowska-Curie Action EpiSeedLink